Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons
نویسندگان
چکیده
Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.
منابع مشابه
Morphological Changes in Hippocampal Ca1 Area in Diabetic Rats: A Golgi-impregnation Study
Background and Objective: Although diabetes mellitus is known to be one of the risk factors for dementia but neuropathic changes in the brain of diabetic patients have not been completely revealed. Therefore, this research study was done to evaluate structural changes in pyramidal neurons of hippocampal ...
متن کاملEffect of Trigonelline on Dendritic Morphology in the Hippocampus and Prefrontal Cortex in Streptozotocin-Induced Diabetic Rats
Introduction: Diabetes mellitus causes adverse changes in the neurological morphology of the hippocampus and prefrontal cortex of the brain by increasing oxidative stress. Trigonelline has antihyperglycemic effects and can inhibit oxidative stress. The aim of this study was to evaluate the protective effect of trigonelline on dendritic changes in hippocampal and prefrontal cortex neurons in dia...
متن کاملREMOD: A Tool for Analyzing and Remodeling the Dendritic Architecture of Neural Cells
Dendritic morphology is a key determinant of how individual neurons acquire a unique signal processing profile. The highly branched dendritic structure that originates from the cell body, explores the surrounding 3D space in a fractal-like manner, until it reaches a certain amount of complexity. Its shape undergoes significant alterations under various physiological or neuropathological conditi...
متن کاملHighly Efficient Transfection of Dendritic Cells Derived from Esophageal Squamous Cell Carcinoma Patient: Optimization with Green Fluorescent Protein and Validation with Tumor RNA as a Tool for Immuno-genetherapy
This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DC...
متن کاملThe unending fascination with the Golgi method
Abstract Introduction In the second half of the 19th century, Camillo Golgi provided a breakthrough staining technique for visualising whole neurons, which are seen as black bodies due to intracellular staining with microcrystalline silver chromate. The high contrast, selective staining properties enabled identification of complete neuronal morphology. This staining technique, termed the Golgi ...
متن کامل